Protocol Validation 2019

Ebola virus

run name date barcodes protocol(s) kit flow cell ID run folder download bulk file download
EBOV Metagenomics 30.08.2019 9=mayinga,
10=kikwit,
11=makona
9n rapid PCR FAK25288 download download
EBOV Metagenomics Smartplex 30.08.2019 9=mayinga,
10=kikwit,
11=makona
smartplex rapid PCR FAK25413 download download
EBOV Amplicons 30.08.2019 3=mayinga,
4=kikwit,
5=makona,
6=negative
multiplex PCR native ligation PCR FAL30025 download download
EBOV Amplicons Flongle 30.08.2019 3=mayinga,
4=kikwit,
5=makona,
6=negative
multiplex PCR native ligation PCR AAQ411 download download

Nipah virus

run name date barcodes protocol(s) kit flow cell ID run folder download bulk file download
NiV Metagenomics 29.08.2019 7=NiV,
8=NiV
7=9n,
8=smartplex
rapid PCR FAK25288 download download
NiV Amplicons 30.08.2019 - smartplex native ligation PCR FAK25288 download download

note: the FAK25288 flow cell was subject to a nuclease flush between runs


Acknowledgements

Thanks to Claire Lonsdale (DSTL), Babak Afrough, Steve Pullan, Miles Carroll and Roger Hewson (PHE) for help with generating these datasets.

Funded by the Wellcome Trust
Collaborators Award 206298/Z/17/Z --- ARTIC network